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KMID : 0368420100530050330
Journal of Plant Biology
2010 Volume.53 No. 5 p.330 ~ p.337
Transcriptomics Analysis Identified Candidate Genes Colocalized with Seed Dormancy QTLs in Rice (Oryza sativa L.)
Qin Huaide

Wu Fuqing
Xie Kun
Cheng Zhijun
Guo Xiuping
Zhang Xin
Wang Jie
Lei Cailin
Wang Jiulin
Mao Long
Jiang Ling
Wan Jianmin
Abstract
Rice seed dormancy is an important trait related to the preharvest sprouting resistance of rice and is controlled by a polygene network. To identify the genes involved in this process, transcriptome analysis was applied to strong seed dormancy indica cultivar N22 and its weak dormancy mutant Q4646. The results showed that 280 genes were significantly upregulated and 244 genes significantly downregulated in the seed of Q4646 as compared to N22 during 25 to 28 days after heading. These genes were mainly involved in stress response, C-compound metabolism, plant development, DNA processing, and lipid metabolism. Some of these genes were colocalized with several reported dormancy QTLs, suggesting that they are possibly candidate genes underlying rice seed dormancy. Our work provides important clues for future effort to clone seed dormant genes in rice.
KEYWORD
Rice, Seed dormancy, Mutant, Microarray, Quantitative trait loci (QTL)
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